Optimized centrifugation for rapid elution of DNA from agarose gels
โ Scribed by Mingyue He; Hong Liu; Yangxi Wang; Brian Austen
- Book ID
- 113409551
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 497 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1050-3862
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๐ SIMILAR VOLUMES
A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovere
We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 an