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Optimized Adaptor Polymerase Chain Reaction Method for Efficient Genomic Walking

โœ Scribed by Peng XU; Rui-Ying HU; Xiao-Yan DING


Book ID
111357699
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
336 KB
Volume
38
Category
Article
ISSN
1672-9145

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only 7 min as the cells started to sediment. The oxidative burst measured for the reaction including Ficoll 400 (Fig. ) similarly started 5 min after the addition of YE but peaked to a maximum of 30 mV (ฯฎ5 mV) after 14 min. Integration of the responses indicated a 5.9ฯซ enhancement in total detected