An Efficient RNA Extraction Method for Estimating Gut Microbial Diversity by Polymerase Chain Reaction

Ultra-rapid, simple, sensitive, and econ

Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction

✍ Dr. Eliane Chungue; Claudine Roche; Marie-France Lefevre; P. Barbazan; Suzanne C 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 566 KB

## Abstract A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties o