A New Approach for Efficient Directional Genome Walking Using Polymerase Chain Reaction

✍ Scribed by M.K. Reddy; Suresh Nair; S.K. Sopory

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 94 KB
• Volume: 306
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2002.5645

✦ Synopsis

only 7 min as the cells started to sediment. The oxidative burst measured for the reaction including Ficoll 400 (Fig. ) similarly started 5 min after the addition of YE but peaked to a maximum of 30 mV (Ϯ5 mV) after 14 min. Integration of the responses indicated a 5.9ϫ enhancement in total detected chemiluminescence. The cells in this reaction stayed in suspension for the duration of the experiment, resulting in a much more accurate measurement of the oxidative burst reaction. The procedure described above allows for the accurate detection of maximum peak heights and represents a significant improvement for the chemiluminescence determination of reactive oxygen species during the oxidative burst in elicitor challenged cells.

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