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Optimization of recombinant protein expression level in Escherichia coli by flow cytometry and cell sorting

✍ Scribed by Elena Soriano; Nicole Borth; Hermann Katinger; Diethard Mattanovich


Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
168 KB
Volume
80
Category
Article
ISSN
0006-3592

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## Abstract In this work, it was discovered that the stability of mRNA in a cell‐free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequ