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Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

✍ Scribed by Jin-Ho Ahn; Taek-Jin Kang; Dong-Myung Kim

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
253 KB
Volume
101
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

In this work, it was discovered that the stability of mRNA in a cell‐free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the controlled abundance of mRNA species, it was possible to manipulate the relative expression level of target proteins by employing the T7 terminator of adjusted stem lengths. Biotechnol. Bioeng. 2008;101: 422–427. © 2008 Wiley Periodicals, Inc.

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