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Optimization of heterologous protein production in Chinese hamster ovary cells under overexpression of spliced form of human X-box binding protein

✍ Scribed by Gulis, Galina; Simi, Kelly Cristina; de Toledo, Renata; Maranhao, Andrea; Brigido, Marcelo


Book ID
125399811
Publisher
BioMed Central
Year
2014
Tongue
English
Weight
804 KB
Volume
14
Category
Article
ISSN
1472-6750

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Overexpression of recombinant proteins in animal cells is commonly achieved by using gene amplification techniques. Gene amplified cells possess up to several thousand genes coding for the target protein. Constitutive expression of these genes leads to high levels of the corresponding mRNA species