Optimization of culture parameters for production of podophyllotoxin in suspension culture ofPodophyllum hexandrum
β Scribed by Saurabh Chattopadhyay; A. K. Srivastava; V. S. Bisaria
- Book ID
- 111634202
- Publisher
- Springer-Verlag
- Year
- 2002
- Tongue
- English
- Weight
- 110 KB
- Volume
- 102-103
- Category
- Article
- ISSN
- 0273-2289
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Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained
In order to improve the production of the cytotoxic lignan podophyllotoxin, seven precursors from the phenylpropanoid-routing and one related compound were fed to cell suspension cultures derived from the rhizomes of Podophyllum hexandrum Royle. These cell cultures were able to convert only coniferi