## Abstract Suspension cultures of Lβ929 fibroblasts grown to densities of 6 to 10 Γ 10^6^ cells/ml through daily centrifugation and resuspension in fresh media, have been maintained for periods up to five months without change in viability or cell size. DNA synthesis and mitosis in these cultures
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Optimization of reovirus production from mouse L-929 cells in suspension culture
β Scribed by Sunghoon Jung; Leo A. Behie; Patrick W. K. Lee; Patrick J. Farrell
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 525 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0006-3592
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Uptake of "-thymidine into suspension cultures of mouse marrow cells was stimulated by the addition of serum-free conditioned medium harvested from cultures of mouse L-cells. Characterization of the "conditioning factor activity" (CFA) by gel filtration, ion exchange chromatography, velocity sedimen