Development of suspension culture ofPodophyllum hexandrumfor production of podophyllotoxin
β Scribed by S. Chattopadhyay; A.K. Srivastava; S.S. Bhojwani; V.S. Bisaria
- Book ID
- 110323304
- Publisher
- Springer Netherlands
- Year
- 2001
- Tongue
- English
- Weight
- 62 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0141-5492
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In order to improve the production of the cytotoxic lignan podophyllotoxin, seven precursors from the phenylpropanoid-routing and one related compound were fed to cell suspension cultures derived from the rhizomes of Podophyllum hexandrum Royle. These cell cultures were able to convert only coniferi
Callus cultures have been established from root explants of aseptically-grown Podophyllum hexandrum seedlings. A fully defined medium based on Gamborg's B5 salts supplemented with 2/4-dichlorophenoxyacetic acid, gibberellic acid and 6-benzylaminopurine was effective for both initiation and sustained