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Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis

✍ Scribed by Tadeusz Wroblewski; Anna Tomczak; Richard Michelmore

Book ID
110948038
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
330 KB
Volume
3
Category
Article
ISSN
1467-7644

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We have investigated factors influencing polyethylene glycol mediated DNA uptake and B-glucuronidase expression in pea (Pisum sativum L.) protoplasts. It was found that for optimal 13-glucuronidase expression the molecular weight and concentration of polyethylene glycol should be 4000 and 20%, respe