Evidence is presented for the introduction of functional copies of the GUS-reporter gene with plastid regulatory signals into chloroplasts after treatment of Nicotiana plumbaginifolia leaf protoplasts with PEG. GUS-activity is found in cells derived from protoplasts treated with PEG in the presence
✦ LIBER ✦
Agrobacterium-mediated transient expression in citrus leaves: a rapid tool for gene expression and functional gene assay
✍ Scribed by Jose F. L. Figueiredo; Patrick Römer; Thomas Lahaye; James H. Graham; Frank F. White; Jeffrey B. Jones
- Book ID
- 106010312
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 819 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0721-7714
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Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt