✦ LIBER ✦

Optimization of polyethylene glycol mediated transient gene expression in pea protoplasts

✍ Scribed by Mogens Nicolaisen; Gert B. Poulsen

Publisher: Springer
Year: 1993
Tongue: English
Weight: 357 KB
Volume: 35
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00043945

No coin nor oath required. For personal study only.

✦ Synopsis

We have investigated factors influencing polyethylene glycol mediated DNA uptake and B-glucuronidase expression in pea (Pisum sativum L.) protoplasts. It was found that for optimal 13-glucuronidase expression the molecular weight and concentration of polyethylene glycol should be 4000 and 20%, respectively. The amount of plasmid DNA should be 25 txg per 5 x 105 protoplasts in each treatment, and the concentration of Mg 2+ in the transformation buffer should be 15 mM. The optimized protocol was applicable to all four pea cultivars tested.

📜 SIMILAR VOLUMES

Transient foreign gene expression in pol

Transient foreign gene expression in polyethylene/glycol treated or electropulsatedEucalyptus gunniiprotoplasts

✍ C. Teulières; J. Grima-Pettenati; C. Curie; J. Teissie; A. M. Boudet 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 734 KB

Conditions for optimal transient gene expression of reporter genes (chloramphenicol acetyl transferase and /3-glucuronidase) by polyethylene glycol or electrical treatment of Eucalyptus gunnii protoplasts derived from callus or cell suspension cultures were investigated. The efficiency of electroper

Transient expression in electroporated p

Transient expression in electroporated pea protoplasts: Elicitor responsiveness of a phenylalanine ammonia-lyase promoter

✍ Tadaaki Hashimoto; Tetsuji Yamada; Akiko Tada; Shinji Kawamata; Yoshikazu Tanaka 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 572 KB

Transient gene expression in tobacco pro

Transient gene expression in tobacco protoplasts: I. Time course of CAT appearance

✍ Markus Pröls; Reinhard Töpfer; Jozef Schell; Hans-Henning Steinbiß 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 592 KB

Efficient transient expression and trans

Efficient transient expression and transformation of PEG-mediated gene uptake into mesophyll protoplasts of pepper (Capsicum annuumL.)

✍ Joo Mi Jeon; Nam Young Ahn; Bo Hwa Son; Cha Young Kim; Chang-deok Han; Gun-Do Ki 📂 Article 📅 2007 🏛 Springer 🌐 English ⚖ 328 KB

Influence of enzyme solution on protopla

Influence of enzyme solution on protoplast culture and transient gene expression in maize (Zea maysL.)

✍ Brigitte Krautwig; Paul A. Lazzeri; Horst Lörz 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 783 KB

The influence of two enzyme solutions, differing only in the presence or absence of Macerozyme, on protoplast yield, colony formation and transient GUS (/~-glucuronidase) activity was studied. For all parameters tested the presence of Macerozyme during protoplast isolation had a negative influence.

Transient gene expression in strawberry

Transient gene expression in strawberry (Fragaria x ananassa Duch.) protoplasts and the recovery of transgenic plants

✍ Marie Nyman; Anita Wallin 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 413 KB

A transient ß-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addit