๐”– Bobbio Scriptorium
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Optimization of a phagocyte microplate chemiluminescent assay

โœ Scribed by Kournikakis, Bill ;Simpson, Maureen

Publisher
John Wiley and Sons
Year
1995
Weight
370 KB
Volume
10
Category
Article
ISSN
0884-3996

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โœฆ Synopsis

Chemiluminescent assays have been used to quantify phagocytic activity since 1972. In recent years these assays have been adapted t o the 96-well microplate format as new luminometers have been developed. In this report w e describe the optimization of a lucigenin enhanced phagocyte chemiluminescent assay using a Titertek Luminoskan. Factors such as cell concentration, serum concentration in the opsonization of the zymosan used and lucigenin concentration were all optimized in our assay. In addition w e have found that some of the unique features of the Luminoskan, continuous microplate agitation during the assay and microplate temperature control up to 43ยฐC. also significantly enhanced the chemiluminescent response.

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