𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Optimization of phagocyte chemiluminescence measurements using microplates and vials

✍ Scribed by Paul Hengster; Marialuise Kunc; Rolf Linke; Thomas Eberl; Wolfgang Steurer; Dietmar Öfner; Fritz Berthold; Raimund Margreiter


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
279 KB
Volume
14
Category
Article
ISSN
1522-7235

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Optimization of a phagocyte microplate c
✍ Kournikakis, Bill ;Simpson, Maureen 📂 Article 📅 1995 🏛 John Wiley and Sons ⚖ 370 KB

Chemiluminescent assays have been used to quantify phagocytic activity since 1972. In recent years these assays have been adapted t o the 96-well microplate format as new luminometers have been developed. In this report w e describe the optimization of a lucigenin enhanced phagocyte chemiluminescent

Measurement of phagocyte chemiluminescen
✍ Blair, Andrea L. ;Cree, Ian A. ;Beck, J. Swanson 📂 Article 📅 1989 🏛 John Wiley and Sons ⚖ 281 KB

The use of chemiluminescence techniques t o study the interaction between bacteria and phagocytes has been useful for examining the extent t o which serum factors, such as opsonins, are important in internalization of the organisms and the response of the cell t o phagocytosed bacteria. However, suc

Measurement of chemiluminescence from ne
✍ Kikuchi, Takashi ;Suzuki, Katsuhiko ;Abe, Tatsuya ;Satoh, Hideki ;Endoh, Tetsu ; 📂 Article 📅 1997 🏛 John Wiley and Sons ⚖ 124 KB

We have developed a microplate photon counting system based on a cooled charge-coupled device (Lumi Box U-800 II) jointly with Maikurotekku Nition Company (Chiba, Japan). The system makes it possible to quantify chemiluminescence (CL) in a 96-well microplate automatically and simultaneously in a sin

Rapid optimization of protein freeze-dry
✍ Yitzchak Grant; Paul Matejtschuk; Paul A. Dalby 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 567 KB

## Abstract Retaining biopharmaceutical proteins in a stable form is critical to their safety and efficacy, and is a major factor for optimizing the final product. Freeze‐dried formulations offer one route for improved stability. Currently the optimization of formulations for freeze‐drying is an em

Measurement of key metabolic enzyme acti
✍ R. Janke; Y. Genzel; A. Wahl; U. Reichl 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 656 KB

## Abstract Sensitive microplate‐based assays to determine low levels of key enzyme activities in mammalian cells are presented. The enzyme platform consists of four cycling assays to measure the activity of 28 enzymes involved in central carbon and glutamine metabolism. The sensitivity limit of al