A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

✍ Scribed by Magrisso, Moni J. ;Bechev, Blagovest G. ;Bochev, Petyo G. ;Markova, Vania I. ;Alexandrova, Margarita L.

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 606 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100203

✦ Synopsis

Luminol chemiluminescence (LCL) is a simple, sensitive and time-saving tool t o elucidate the oxidative activity o f polymorphonuclear leukocytes (PMNL). In this study, a new approach for analysing the LCL kinetics, recorded from stimulated PMNL, and for a more accurate elucidation o f their functional state, is proposed. This approach is based on the proposal that the LCL kinetics o f stimulated PMNL is a result o f timeprobabilistic nature o f the processes, leading t o light emission. On this basis the LCL response was described by the parameters o f a Poisson-type distribution.

Relationships between these parameters and some factors influencing LCL kinetics (number o f PMN L and erythrocytes, temperature, stirring) were investigated. The model descriptions o f the LCL responses obtained had t w o components (modes). Their development in time was different. It was suggested, that the t w o phases o f the model LCL response were connected with extracellular and intracellular LCL. The terms and the advantages of this approach for analysis o f phagocyte oxidative capacity are discussed.