✦ LIBER ✦

Automation and computerization of chemiluminescence: A new methodological approach in the study of human phagocytes

✍ Scribed by Parente, R. ;Melotti, C.

Publisher: John Wiley and Sons
Year: 1989
Weight: 302 KB
Volume: 4
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170040179

No coin nor oath required. For personal study only.

✦ Synopsis

Peripheral blood phagocytic cells (PMNLs) are activated by contact with opsonized particles. Metabolic activation of PMNLs is associated with a remarkable increase in the respiratory burst and generates high energy oxygen compounds which are responsible for the bactericidal activity of PMNLs and for their ability t o produce luminol-dependent chemiluminescence (CL). The CL phenomenon is measured by an automated and computerized photoluminometer (Berthold LB950) in whole blood stimulated with opsonized zymosan.

This whole blood method of CL measurement has been applied t o the study of the phagocytic process and t o the investigation of cellular and humoral abnormalities in several pathologies, indicating this assay as a simple, rapid and reliable test.

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