Optimization of a Fed-batch Process for Production of a Recombinant Antibody
β Scribed by D. K. ROBINSON; T. C. SEAMANS; S. L. GOULD; D. J. DiSTEFANO; C. P. CHAN; D. K. LEE; T. BIBILA; K. GLAZOMITSKY; S. MUNSHI; B. DAUGHERTY; L. O'NEILL PALLADINO; J. STAFFORD-HOLLIS; G. F. HOLLIS; M. SILBERKLANG
- Book ID
- 119871053
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 539 KB
- Volume
- 745
- Category
- Article
- ISSN
- 0890-6564
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A fed-batch cell culture process was developed that has general applicability to all evaluated Sp2/0 (n = 8) and NS0 (n = 1) antibody-producing cell lines. The two key elements of this generic process were a protein-free concentrated feed medium, and a robust, metabolically responsive feeding strate
in the column enter. EMP permits to introduce the flow by a square-wave disturbance, avoiding back mixing close to plug flow. The chosen pulsation frequencies were determined in a previous study. Residence time distribution (RTD) analysis was calculated by step introducing a tracer into the column