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Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses

✍ Scribed by Lin, Yun; Gallardo, Humilidad F.; Ku, Geoffrey Y.; Li, Hao; Manukian, Gregor; Rasalan, Teresa S.; Xu, Yinyan; Terzulli, Stephanie L.; Old, Lloyd J.; Allison, James P.; Houghton, Alan N.; Wolchok, Jedd D.; Yuan, Jianda


Book ID
122798338
Publisher
Informa plc
Year
2009
Tongue
English
Weight
503 KB
Volume
11
Category
Article
ISSN
1465-3249

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## Abstract Spontaneous tumors frequently express antigens that can be recognized by the immune system but nevertheless manage to evade immune surveillance. To better understand the mechanism of evasion, we followed CD8 and CD4 T cells reacting against a subcutaneously growing tumor, modified to ex