𝔖 Bobbio Scriptorium
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Optical Sectioning Microscopy: Cellular Architecture in Three Dimensions

✍ Scribed by Agard, D A

Book ID
121419015
Publisher
Annual Reviews
Year
1984
Tongue
English
Weight
1011 KB
Volume
13
Category
Article
ISSN
0883-9182

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## Abstract We present a new beam‐shaping technique for two‐color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded‐ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such m