Optical-sectioning improvement in two-color excitation scanning microscopy

✍ Scribed by Cristina Ibáñez-López; Isabel Escobar; Genaro Saavedra; Manuel Martínez-Corral

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 261 KB
• Volume: 64
• Category: Article
• ISSN: 1059-910X
• DOI: 10.1002/jemt.20073

✦ Synopsis

Abstract

We present a new beam‐shaping technique for two‐color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded‐ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point‐spread‐function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation. Microsc. Res. Tech. 64:96–102, 2004. © 2004 Wiley‐Liss, Inc.