Optical-sectioning improvement in two-co
β
Cristina IbÑñez-LΓ³pez; Isabel Escobar; Genaro Saavedra; Manuel MartΓnez-Corral
π
Article
π
2004
π
John Wiley and Sons
π
English
β 261 KB
## Abstract We present a new beamβshaping technique for twoβcolor excitation fluorescence microscopy. We show that by simply inserting a properly designed shadedβring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such m