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Ontogenetic development of [3H]naloxone binding in rat brain

✍ Scribed by J.T. Coyle; Candace B. Pert

Book ID
118953608
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
709 KB
Volume
15
Category
Article
ISSN
0028-3908

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The sulfhydryl alkylating reagent N-ethylmaleimide (NEM) blocks opioid receptor binding and receptor/G-protein coupling. Sodium partially restores [ 3 H]naloxone binding after inhibition by NEM to reveal sodium-dependent [ 3 H]naloxone sites, defined as binding in the presence of 50-100 mM NaCl afte