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Binding of 3H-naloxone in the mouse brain: Effect of ions and tolerance development

✍ Scribed by Robert J. Hitzemann; Barbara A. Hitzemann; Horace H. Loh

Book ID
118936397
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
430 KB
Volume
14
Category
Article
ISSN
0024-3205

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The sulfhydryl alkylating reagent N-ethylmaleimide (NEM) blocks opioid receptor binding and receptor/G-protein coupling. Sodium partially restores [ 3 H]naloxone binding after inhibition by NEM to reveal sodium-dependent [ 3 H]naloxone sites, defined as binding in the presence of 50-100 mM NaCl afte