𝔖 Bobbio Scriptorium
✦   LIBER   ✦

One step purification procedure of elastase from pancreatic powder by affinity chromatography

✍ Scribed by Kenji Katagiri; Toshihiko Takeuchi; Keiko Taniguchi; Makoto Sasaki

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
582 KB
Volume
86
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Pancreatic elastase was isolated from acetone powder of porcine pancreas by a one step purification procedure on a trialanyl CH-Sepharose 4B affinity column. This column exhibited affinity not only for active elastase but also for trypsin and chymotrypsin which were present in the same pancreatic powder. However, as the extent of affinity toward elastase is considerably higher, the proper conditions were determined with which the adsorbed elastase was isolated in a highly purified form. The yield of elastolytic activity ranged from 60-85% and the purified elastase was shown to be one component by polyacrylamide disc electrophoresis.

📜 SIMILAR VOLUMES

A single-step purification of rat pancre
A single-step purification of rat pancreatic and salivary amylase by affinity chromatography
✍ Peter H. Burrill; Patsy M. Brannon; Norman Kretchmer 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 263 KB

Rat pancreatic and salivary amylase [( 1 -4) a-D-gkan glucanohydrolase] (EC 3.2.1.1) were purified 20-to 50-fold with a yield above 75% by a single-step affinity chromatographic procedure. The affinity ligand was an a-glucohydrolase inhibitor (proprietary name Bay g5421) coupled to w-aminohexyl-Seph

One-Step Purification of Bovine Adrenal
One-Step Purification of Bovine Adrenal Glucose-6-phosphate Dehydrogenase by Affinity Chromatography
✍ Ernst Bürgisser; Jean-Luc Fauchère 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 German ⚖ 456 KB

## Abstract Das konventionelle Reinigungsverfahren von Glucose‐6‐phosphat‐dehydrogenase (G6PDH) (E.C. 1.1.1.49) aus Rinder‐Nebennierenrinde wird durch eine einzige affinitätschromatographische Trennungsstufe ersetzt. Die Anwendung einer mit Nicotinamid‐Adenin‐Dinukleotid‐Phosphat (NADP) substituier

One step purification of flavanone glyco
One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography
✍ Chul Young Kim; Hee Ju Lee; Mi Kyeong Lee; Mi-Jeong Ahn; Jinwoong Kim 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 994 KB

## Abstract Flavanone glycosides were successfully separated from the crude extract of __Poncirus trifoliata__ by preparative centrifugal partition chromatography with a two‐phase solvent system composed of ethyl acetate–acetonitrile–water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg),

A two-step purification of cytochrome P-
A two-step purification of cytochrome P-450 from adult pig testis by pregnenolone affinity column chromatography
✍ Masahiro Kuwada 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 141 KB

Adult testicular cytochrome P-450 was purified by a two-step procedure utilizing hydroxylapatite and pregnenolone affinity column chromatography. The cytochrome P-450 was determined to have an isoelectric point of 6.43 on analytical isoelectric focusing. The purified cytochrome P-450 was found to be