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One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography

✍ Scribed by Chul Young Kim; Hee Ju Lee; Mi Kyeong Lee; Mi-Jeong Ahn; Jinwoong Kim

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 994 KB
Volume: 30
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200700168

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✦ Synopsis

Abstract

Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two‐phase solvent system composed of ethyl acetate–acetonitrile–water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by ^1^H NMR, ^13^C NMR, and ESI‐MS.

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