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One-step purification of soluble recombinant human 6-phosphogluconate dehydrogenase from Escherichia coli

โœ Scribed by Chan, Barden; Sukhatme, Vikas P.

Book ID
122905267
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
947 KB
Volume
92
Category
Article
ISSN
1046-5928

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Purification of human 6-phosphogluconate
Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation : Simultaneous purification of lactate dehydrogenase
โœ Y. Kroviarski; S. Cochet; C. Vadon; A. Truskolaski; P. Boivin; O. Bertrand ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 429 KB

The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharos