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Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation : Simultaneous purification of lactate dehydrogenase

✍ Scribed by Y. Kroviarski; S. Cochet; C. Vadon; A. Truskolaski; P. Boivin; O. Bertrand

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 429 KB
Volume: 449
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)94404-2

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✦ Synopsis

The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharose. This technique also gave purified lactate dehydrogenase. Both enzymes showed single bands in SDS polyacrylamide gel electrophoresis.