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Overexpression in Escherichia coli and Purification of the 6-Phosphogluconate Dehydrogenase of Trypanosoma brucei

โœ Scribed by M.P. Barrett; C. Phillips; M.J. Adams; R.W.F. Lepage


Book ID
115644797
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
529 KB
Volume
5
Category
Article
ISSN
1046-5928

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6-Phosphogluconate dehydrogenase in cell
โœ Sly, L. I. ;Doelle, H. W. ๐Ÿ“‚ Article ๐Ÿ“… 1968 ๐Ÿ› Springer-Verlag โš– 496 KB

Investigations into the properties of 6-PG dehydrogenase in cell free extracts ofEscherichia coli revealed a pit optimum at pH 9.5 with a sharp decline on both sides of the optimum. The addition of 1.0 โ€ข 10 -3 ~ MgCI 2 produced maximal activity, whereas higher concentrations caused inhibition. The K