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Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase

✍ Scribed by Katy Montin; Carlo Cervellati; Franco Dallocchio; Stefania Hanau


Book ID
111312188
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
271 KB
Volume
274
Category
Article
ISSN
1432-1327

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The binding of a series of low-molecular-mass, active-site-directed thrombin inhibitors (399 -575 Da) to human ␣-thrombin was investigated by surface plasmon resonance technology (BIACORE), stopped-flow spectrophotometry, and isothermal titration microcalorimetry (ITC). The equilibrium constants K D