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On the Salt-Induced Activation of Lyophilized Enzymes in Organic Solvents: Effect of Salt Kosmotropicity on Enzyme Activity

✍ Scribed by Ru, Michael T.; Hirokane, Scott Y.; Lo, Andy S.; Dordick, Jonathan S.; Reimer, Jeffrey A.; Clark, Douglas S.

Book ID
121337037
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
116 KB
Volume
122
Category
Article
ISSN
0002-7863

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Optimizing the salt-induced activation o
Optimizing the salt-induced activation of enzymes in organic solvents: Effects of lyophilization time and water content
✍ Michael T. Ru; Jonathan S. Dordick; Jeffrey A. Reimer; Douglas S. Clark πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 171 KB πŸ‘ 2 views

The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resultin