Improving Activity of Salt-Lyophilized Enzymes in Organic Media
โ Scribed by Abhijeet P. Borole; Brian H. Davison
- Book ID
- 107564648
- Publisher
- Springer-Verlag
- Year
- 2007
- Tongue
- English
- Weight
- 95 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0273-2289
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resultin
A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme prepara