On the question of phosphate complexes of osmium tetroxide: kinetics of reactions with nucleotides and dinucleoside monophosphates

✍ Scribed by Robert L. Clark; E.J. Behrman

• Publisher: Elsevier Science
• Year: 1976
• Weight: 117 KB
• Volume: 5
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80296-3

✦ Synopsis

Konishevskaya et al. [I] observed that phosphate ions increased the rate of the reaction between hydrazine and vanadate ions catalyzed by osmium tetroxide. They claimed that phosphate ions form a 1:l complex with osmium tetroxide, & = 250 + 60. We were concerned with this report because of our interest 12-41 in the reaction between osmium tetroxide and the olefinic group of various nucleic acid derivatives which contain ionized phosphate groups and also because we have generally used phosphate buffers in our kinetic work. Table 1 summarizes our present results. The data show that sodium phosphate produces moderate rate increases in the reaction between the osmium tetroxide-pyridine system and the olefinic group in thymine derivatives in agreement with the data of Konishevskaya et al. [ 11 on the hydrazine-vanadate system. We are in