On the Mechanism of Toxin Resistance in Cell Variants Resistant to Abrin and Ricin
β Scribed by Sjur OLSNES; Karin REFSNES
- Book ID
- 115116766
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 907 KB
- Volume
- 88
- Category
- Article
- ISSN
- 1432-1327
No coin nor oath required. For personal study only.
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The role of impaired toxin uptake in conferring cellular resistance t o the plant toxin RCAII (ricin) has been examined using a murine BW5 147 lymphoma line and a toxin-resistant variant (BW5 147RicR.3) selected by repeated exposure t o RCAII. The toxin-resistant variant is 250 times more resistant
The toxic lectin modeccin, which inhibits protein synthesis in eukaryotic cells, is cleaved upon treatment with 2-mercaptoethanol into two peptide chains which move in polyacrylamide gels at rates corresponding to molecular weights 28,000 and 38,000. After reduction, the toxin loses its effect on ce