𝔖 Bobbio Scriptorium
✦   LIBER   ✦

On-line size-exclusion chromatography/mass spectrometry of low molecular mass heparin

✍ Scribed by Jens Henriksen; Lene Hoffmeyer Ringborg; Peter Roepstorrf

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
374 KB
Volume
39
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Heparin and low molecular mass heparin (LMMH) consists of complex mixtures of sulphated linear oligosaccharides that are difficult to analyse. An on‐line size exclusion chromatographic/electrospray ionization (ESI) mass spectrometric method that allows the determination of more than 60 components in an LMMH preparation is presented. The experimental setup includes on‐line cation exchange in order to prevent massive adducting in the ESI interface. Copyright © 2004 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

On-line size-exclusion chromatography/el
On-line size-exclusion chromatography/electrospray ionisation mass spectrometry of aquatic humic and fulvic acids
✍ Linn Persson; Tomas Alsberg; Gyula Kiss; Göran Odham 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB

Isolated aquatic humic and fulvic acids were analysed with on-line size exclusion chromatography/electrospray ionisation mass spectrometry (SEC/ESI-MS). An eluent composition which enabled electrospray ionisation was identified. The SEC separation improved interpretability of mass spectra and may op

Molecular mass calibration in size-exclu
Molecular mass calibration in size-exclusion chromatography of coal derivatives
✍ Keith D. Bartle; Michael J. Mulligan; Norman Taylor; Terence G. Martin; Colin E. 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 580 KB

A number of procedures have been investigated for the calibration of a size-exclusion chromatography (s.e.c.) column in the determination of molecular mass (MM) distributions of coal derivatives. The behaviour of narrow fractions of coal extracts in the MM range 2003000 was compared with a variety o

Multidimensional chromatography using on
Multidimensional chromatography using on–line coupled microcolumn size exclusion chromatography–capillary gas chromatography–mass spectrometry for determination of polymer additives
✍ Hernan J. Cortes; Bruce M. Bell; Curt D. Pfeiffer; Jeffrey D. Graham 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 581 KB

A multidimensional system consisting of microcolumn size exclusion chromatography coupled on-line with capillary gas chromatography and utilizing mass spectrometry for the rapid identification of polymer additives is described. Applicability of the system was demonstrated for a broad spectrum of com

Analysis of nucleic acids by on-line liq
Analysis of nucleic acids by on-line liquid chromatography–Mass spectrometry
✍ Christian G. Huber; Herbert Oberacher 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 469 KB

## Abstract | I. | Introduction | 311 | | | A. Nucleic Acid Structures and Properties | 311 | | | B. Principles of LC of Nucleic Acids | 312 | | |   1. Reversed‐Phase HPLC | 312 | | |   2. Ion‐Pair Reversed‐Phase HPLC | 314 | | |   3. Affinity Chromatography | 314 | | | C. Principles of ESI M