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On-line SFE-GC for determination of PCBs in human milk and blood serum

✍ Scribed by Johansen, Hege Rebecka ;Thorstensen, Christian ;Greibrokk, Tyge ;Becher, Georg

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
438 KB
Volume
16
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

On‐line coupled supercritical fluid extraction and gas chromatography (SFE‐GC) has been utilized for the determination of PCBs and other organochlorine compounds in human milk and blood serum. The procedure involved preconcentration of the sample on C~18~‐silica sorbent in an extraction cell: after precipitation of the proteins up to 20 ml of human milk was concentrated on 0.5 g of sorbent. Serum (up to 5 ml) was applied to the C~18~ material without pretreatment. Basic alumina was utilized as a selective adsorbent for lipids in the on‐line SFE‐GC system. The method was used to analyze milk and serum spiked with 0.5 and 10 ng of Aroclor 1260 and the results compared with those obtained by liquid–liquid extraction of serum.

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