✦ LIBER ✦

High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum

✍ Scribed by Anniek Schellen; Bert Ooms; Mark van Gils; Otto Halmingh; Erik van der Vlis; Dick van de Lagemaat; Elwin Verheij

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 49 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(20000229)14:4<230::aid-rcm872>3.0.co;2-j

No coin nor oath required. For personal study only.

✦ Synopsis

The feasibility of high throughput on-line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns.

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