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Observation of Tetrahydrofolylpolyglutamic Acid in Bacteria Cells by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

✍ Scribed by Randy J. Arnold; James P. Reilly

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
151 KB
Volume
281
Category
Article
ISSN
0003-2697

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✦ Synopsis

Tetrahydrofolylpolyglutamic acid in whole bacteria cells and cell lysates is analyzed by matrix-assisted laser desorption/ionization mass spectrometry. The speed, mass information, and tolerance to impurities of this technique make it ideal for monitoring the glutamation levels of folic acid in biological systems. Folylpolyglutamic acid is observed in a few strains of E. coli and two species of Staphylococcus bacteria. The effects of growth time, growth media, and the addition of methotrexate, a dihydrofolate reductase inhibitor, are also studied.

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