Rapid Identification of Bacteria by Direct Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Whole Cells

✍ Scribed by Thaiya Krishnamurthy; Philip L. Ross

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 534 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(199612)10:15<1992::aid-rcm789>3.0.co;2-v

✦ Synopsis

Several characteristic ions were observed during the direct analysis of a variety of both gram-negative and grampositive intact bacterial cells by the matrix-assisted laser desorptionlionization mass spectrometry (MALDI-MS) technique. The entire process, involving absolutely no sample processing, could be completed in less than ten minutes. A number of specific biomarkers, generated reproducibly for each type of cell from the corresponding mass spectrum, permitted the identification, as well as the distinction, of pathogenic bacteria from their nonpathogenic counterparts. In addition, individual strains of a specific organism could also be differentiated easily. Some of these biomarkers correspond to those observed earlier during the MALDI-MS analysis of protein extracts of the same bacteria. This approach, which can yield valuable data for rapid classification and detection of microorganisms, represents a substantial breakthrough for rapid screening of environmental as well as biological samples.