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Observation of Non-covalent Interactions Between Beauvericin and Oligonucleotides Using Electrospray Ionization Mass Spectrometry

✍ Scribed by Gabriella Pócsfalvi; Giuseppe Di Landa; Pasquale Ferranti; Alberto Ritieni; Giacomino Randazzo; Antonio Malorni

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
236 KB
Volume
11
Category
Article
ISSN
0951-4198

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✦ Synopsis

Electrospray ionization mass spectrometry has been used to study the possible non-covalent interaction between oligonucleotides and beauvericin (B) mycotoxin. Beauvericin-oligonucleotide adduct formation was observed even at low mycotoxin concentration (25 pmol/microL). Adducts were found with different numbers of B ligands attached. The selectivity of binding of B ligands to two different oligonucleotides has been shown to be similar indicating that beauvericin does not have a strongly preferred base sequence or base site in the DNA. In a competitive complexation reaction, beauvericin forms specific adducts with oligonucleotide while another mycotoxin, nigeromicin, which causes apoptosis without fragmentation of DNA, does not.

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