Although protein is commonly stated to interfere in the determination of carbohydrate using sulfuric acid solutions of anthrone, such tryptopban-free proteins as insulin, and mixtures of amino acids containing no tryptophan, give little color during short heating periods with the reagent. The small
β¦ LIBER β¦
o-BENZOQUINONE-A REAGENT FOR DETERMINING CONFORMATIONAL DIFFERENCES IN RELATED PROTEINS
β Scribed by GUPTA, MUNISHWAR N. ;MURTHY, G.S. ;VITHAYATHIL, PAUL J.
- Book ID
- 115097596
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Tongue
- English
- Weight
- 276 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0367-8377
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Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei