Nutrition of animal cells in tissue culture. III. Effect of ethyl alcohol on cell survival and multiplication

✍ Scribed by Parker, Raymond C. ;Morgan, Joseph F. ;Morton, Helen J.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1950
• Tongue: English
• Weight: 536 KB
• Volume: 36
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030360307

✦ Synopsis

During the past three years, a series of studies has been made in this hboratory in an attempt to prepare a medium of chemically-known composition that will support the continuous multiplication of animal cells in tissue culture. These studies have resulted in the development of synthetic Mixture No. 199 (Morgan, Morton and Parker, '50) that supports cell life for approximately 5 weeks. 111 the course of this work, it was considered advisable to add to the mixture a member of the Tween series of surfaoe-active agents, the Tween to serve as a source of water-soluble fatty acid and as a means of dissolving fat-soluble compounds. Before Vitamins A and D, and cholesterol, could be added to the mixture, it was found necessary t o prepare an alcoholic stock solution of these substances and to diluhe this with Tween. This combination gave a clear solution even when highly diluted in the aqueous medium. It mas observed, however, that in the presence of certain concentrations of Tween and alcohol the survival time of the cultures was shortened (Morton, Morgan and Parker, '50). Whcn a minimal amount of alcohol (0.01%) w-as used, this effect was eliminated and Twcen could he incorporated at This investigation was supported, in pai-t, by grants from the National Cancer Institiite of Canada and the Ontario Cancer Treatment and Research Foundation.

'Grateful acknowledgment is made to Mi% 0. E. Roekett and hlr. C. J. MacFayden for technical assistance.