Nutrition of animal cells in tissue culture. V. Effect of initial treatment of cultures on their survival in a synthetic medium

✍ Scribed by Morton, Helen J. ;Morgan, Joseph F. ;Parker, Raymond C.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1951
• Tongue: English
• Weight: 570 KB
• Volume: 38
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030380307

✦ Synopsis

Previous studies from this laboratory have reported the dcrelop~iient of a synthetic feeding mixture (no. 199) that supports cell life for a n avemge period of 4 to 5 weeks (Morgan, Morton a i d Parker, '50 ; Norton, Norgaii and Parker, ' .50 ; Parker, Xorgan and Morton, '50). I n the experiments described, chick embryo mcseiichyrne tissues were cnltivated dii*cctly 011 the iiiiier surface of pyrex test tubes, witliout the use of fibrin clots, and the feeding mixtures wei-e reiicwed at frequent interrals. Tlie transfer of nutrient substaiices from the embryos was reduced to a minimum hy tlie use of the siiiallest amount of tissue that would provide adequate growth areas. In the early experiments, the cultures were treated with a preliimiiiary feeding solution containing horse serum and chick enilwyo extract, which was left on the cultures for three clays while the cells were becoming established. On the 4th day, tlie preliminary feeding nlixture was removed and i.eplacec1 by a synthetic medium. All cultures mcre then maintained in syiithetic mcdia until living cells v7ere 110 longer tli scei*nible.

Thi.; inwstigntion mas supported, in part, by grants from tlir Sational Cancer Institute of Canada and the Ontario Cancer Treatment amd Research I"oundation.

2TVith the terliiiicnl assistance of Miss B. .J. Rarrett, JIiss F,. A . Burr and Miss 0. l?. Rockctt.