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Effect of ribonuclease and neuraminidase on the electrophoretic mobility of tissue culture cells in parasynchronous growth

✍ Scribed by E. Mayhew

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
372 KB
Volume
69
Category
Article
ISSN
0021-9541

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✦ Synopsis

Cultured mammalian cells (RPMI no. 41) in parasynchronous growth

were treated, at different stages of the mitotic cycle, with neuraminidase and ribonuclease, separately and sequentially, and their electrophoretic mobilities determined. Changes in the electrophoretic mobility of these cells are probably mainly due to variations in the density of negatively charged groups susceptible to neuraminidase, although variations in groups susceptible to ribonuclease may occur. It is suggested that the observed variations in electrophoretic mobility of different cells may be due to differences in the relative lengths of different lifecycle phases. Where G2 phase is relatively long or GI relatively short the cell populations will have higher mean electrophoretic mobilities.

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