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Inhibitory Effect of (−)-Epicatechin 4-Benzylthioether on the Growth of Glioma Cells in Culture

✍ Scribed by Stephen Shih-Fan Hsu; Shuo-Bin Jou; Feng-Lin Hsu; Tzu-Feng Yang; Juei-Tang Cheng

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
367 KB
Volume
10
Category
Article
ISSN
0951-418X

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✦ Synopsis

The effect of ( -)-epicatechin 4-benzylthioether (EC-BE) on the growth of cultured rat glioma cells was investigated. EC-BE was found to produce a concentration-dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with ( -)-epicatechin acetoxymethyl ester (EC-AM). The original compound ( -)-epicatechin, which gave rise to EC-AM and EC-BE, failed to produce a similar result. Because both EC-BE and EC-AM are membrane permeable, while ( -)-epicatechin is not, the results indicated that the ( -)-epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC-BE inhibited the incorporation of [3H]thymidine into DNA while the uptake of extracellular ['Hlthymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC-BE. These data suggest that EC-BE can inhibit the growth of glioma cells by aecting a redox pathway related to the formation of DNA.

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