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Nucleotide sequence of the chloroplast 23S rRNA gene from alder (Alnus incana)

✍ Scribed by Madeleine Lévesque; Douglas A. Johnson

Publisher
Springer
Year
1992
Tongue
English
Weight
126 KB
Volume
18
Category
Article
ISSN
0167-4412

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✦ Synopsis

From an alder (Alnus incana) genomic library, we have isolated a clone containing the entire chloroplast rRNA operon. Restriction fragments spanning the 23S rRNA gene were subcloned into M 13mp 19, deletions were prepared [ 1 ] and both strands were sequenced [ 3]. The complete nucleotide sequence (2811 bp) is shown in Fig. 1.

The 23 S coding region has a G + C content of 55~o and shows 98.3~o homology to the corresponding gene from Nicotiana tabacum [ 5 ], also a member of the subclass Magnoliopsida (dicots), and 95.3~ homology to the 23S rRNA gene of Zea mays [2] a member of the subclass Liliopsida (monocots). A 68 bp sequence thought to be specific to monocots [4] is absent from the alder sequence.

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