Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli
β Scribed by Miles, John S.; Guest, John R.
- Book ID
- 121444313
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 909 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0378-1119
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We report here the complete nucleotide sequence of the E. coli triose phosphate isomerase gene. The gene encodes a polypeptide of 255 amino acids which is approximately 46% homologous to eukaryotic triose phosphate isomerases, and approximately 38% homologous to the enzyme from a thermophilic bacter
The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain