Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12
β Scribed by Paul E. STEPHENS; Hilary M. LEWIS; Mark G. DARLISON; John R. GUEST
- Book ID
- 115122011
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 856 KB
- Volume
- 135
- Category
- Article
- ISSN
- 1432-1327
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The hemB gene of Escherichia coli K12, coding for porphobilinogen synthase (PBG-S; syn., 5-aminolevulinic acid dehydratase, ALA-D), was cloned following insertion of an EcoRI fragment of plasmid F'13 into the mobilizable vector pCR1. The hybrid plasmid carrying the hemB gene was able to complement a
The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain