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Nuclease activity of 1,10-phenanthroline-copper ion. Chemistry of deoxyribose oxidation

✍ Scribed by Goyne, Thomas E.; Sigman, David S.

Book ID
118743625
Publisher
American Chemical Society
Year
1987
Tongue
English
Weight
395 KB
Volume
109
Category
Article
ISSN
0002-7863

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Conformational analysis of lac promoters
Conformational analysis of lac promoters using the nuclease activity of 1,10-phenanthroline-copper ion
✍ David S. Sigman; Annick Spassky; Sylvia Rimsky; Henri Buc πŸ“‚ Article πŸ“… 1985 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 826 KB

The artificial nuclease activity of (OP),Cu+ and H202 has been used to analyze the DNA conformation of single-stranded DNA from the coliphage M-13, and the B-type DNA of the promoter region of the lac operon. Despite the strong kinetic preference of the reaction of B-DNA, demonstrated with synthetic