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Nuclear DNA content, proliferating-cell nuclear antigen (PCNA) and p53 immunostaining in predicting progression of laryngeal cancer in situ lesions

✍ Scribed by Eva Munck-Wikland; Staffan Edström; Eva Jungmark; Richard Kuylenstierna; Johan Lindholm; Gert Auer

Publisher
John Wiley and Sons
Year
2007
Tongue
French
Weight
495 KB
Volume
56
Category
Article
ISSN
0020-7136

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✦ Synopsis

Squamous epithelial cancer in situ (CIS) of the upper aerodigastric tract is a histopathologically well-defined condition. However, in clinical practice, morphological grading of dysplasia is difficult and shorn large variability. The biology of CIS remains eni tic, and there is yet no reliable way to predict whether a K l e s i o n will progress to imarive cancer, remain stable or regress. In the search for markers able to foretell clinical outcome, we performed image DNA qtometry (ICM) and immunohistochemical staining for PCNA as well as p53 in 38 laryngeal CIS lesions, of which 9 progressed to invasive cancer. The majority of the CIS lesions displayed high-grade DNA aberration, a high PCNA-positive rate, and every third lesion was p53-positive by immunostaining. The lesions which progressed to invasive cancer showed a clear tendency towards more pronounced DNA aberration, a higher percentage of intense PCNA staining and more frequent p53 positivity. By combining the results from the analyses of DNA, PCNA and p53 in a prognostic index for each individual case, we correctly classihed-82% of the lesions as progressors or non-progresso&.

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